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Products / Wound and antibiotic resistance

Wound and antibiotic resistance

Pongo Antibiotic Resistance Panel A

24 antibiotic resistance genes, run on the same extract as your pathogen panel.

For Research Use Only. Not for use in diagnostic procedures.

Overview

Qualitative multiplex detection of antibiotic resistance genes found in bacteria. It is designed to run on the same extracted sample as the Glyphis Wound and Lioness UT panels, so labs get pathogen and resistance results from one extraction.

Targets

Beta-lactamases and carbapenemases

  • KPC (blaKPC)
  • NDM-1
  • OXA-48 (blaOXA)
  • VIM (blaVIM)
  • IMP-7 (blaIMP)
  • CTX-M Group 1
  • CTX-M Group 2
  • CTX-M Group 9
  • SHV
  • TEM
  • ACT
  • ampC
  • FOX

Other resistance genes

  • mecA
  • vanA
  • vanB
  • qnrA
  • qnrB
  • qnrS
  • ermA
  • ermB
  • sul1
  • sul2
  • tet(A)

Specifications

FormatqPCR, qualitative multiplex
Primer and probe mix6 tubes
Positive controls3 tubes, included
FluorophoresFAM, HEX, Cy5, ROX
Sample inputDNA extracted from bacterial samples. Performance to be verified by the user.
Recommended master mixSeqOnce™ qPCR Multiplex Amplification Kit 2400, PN 500022. Sold separately.
Compatible instrumentsInstruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including:
Bio-Rad CFX96 / CFX384 and CFX Opus
Roche LightCycler 480
Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage−20 °C

Ordering

ProductSKUReactions
Pongo Antibiotic Resistance Panel A50000348
SeqOnce™ qPCR Multiplex Amplification Kit 240050002248
Panel and master mix bundle500003 + 50002248

Product information sheets are available on request.