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Products / Wound and antibiotic resistance

Wound and antibiotic resistance · Combo panel

Glyphis+ Wound/ABR Combo Panel

Glyphis Wound Panel A and Pongo Antibiotic Resistance Panel A in one kit: wound pathogens and 24 resistance genes from one extract.

For Research Use Only. Not for use in diagnostic procedures.

SeqOnce kit by Bandit Biosystems, Glyphis+ Wound/ABR Combo Panel: carton and reagent tubes
Representative kit packaging. Tube count and labels vary by panel and format.

Overview

The Glyphis+ Wound/ABR Combo Panel pairs the Glyphis Wound Panel A (aerobic, anaerobic and yeast targets plus an internal control) with the Pongo Antibiotic Resistance Panel A (24 resistance genes). Both panels run on the same extracted DNA, so labs get wound pathogen and resistance results from one extraction.

Includes the full Glyphis Wound Panel A and Pongo Antibiotic Resistance Panel A, with positive controls for each.

Targets

Glyphis Wound Panel A

Aerobic and facultative bacteria

  • Acinetobacter baumannii
  • Citrobacter freundii
  • Enterobacter cloacae
  • Enterobacter (Klebsiella) aerogenes
  • Enterococcus faecalis
  • Enterococcus faecium
  • Escherichia coli
  • Kingella kingae
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus mirabilis
  • Proteus vulgaris
  • Pseudomonas aeruginosa
  • Serratia marcescens
  • Staphylococcus aureus
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Streptococcus pyogenes (Group A)

Anaerobes

  • Bacteroides fragilis
  • Clostridium novyi
  • Clostridium perfringens
  • Clostridium septicum

Yeasts

  • Candida albicans
  • Candida auris
  • Candida glabrata
  • Candida krusei
  • Candida lusitaniae
  • Candida parapsilosis
  • Candida tropicalis

Internal control

  • Human RNase P (hRPP)

Pongo Antibiotic Resistance Panel A

Beta-lactamases and carbapenemases

  • KPC (blaKPC)
  • NDM-1
  • OXA-48 (blaOXA)
  • VIM (blaVIM)
  • IMP-7 (blaIMP)
  • CTX-M Group 1
  • CTX-M Group 2
  • CTX-M Group 9
  • SHV
  • TEM
  • ACT
  • ampC
  • FOX

Other resistance genes

  • mecA
  • vanA
  • vanB
  • qnrA
  • qnrB
  • qnrS
  • ermA
  • ermB
  • sul1
  • sul2
  • tet(A)

Specifications

FormatqPCR, qualitative multiplex
Panels includedGlyphis Wound Panel A and Pongo Antibiotic Resistance Panel A
Primer and probe mix15 tubes
Kit size48 reactions of each of the 15 primer and probe mixes
Larger kits500 to 1,000 reaction kits are available as a custom order. Request a quote.
Positive controls7 tubes, included (4 Glyphis, 3 Pongo)
FluorophoresFAM, HEX, Cy5, ROX
Sample inputDNA extracted from swab samples using a method that recovers bacterial and fungal DNA. Performance to be verified by the user.
Reaction volume15 µL, in 96- or 384-well plates
Run timeLess than 60 minutes (Glyphis); approximately 60 minutes (Pongo)
Recommended master mixSeqOnce™ qPCR Multiplex Amplification Kit 3600, PN 500024, and SeqOnce™ qPCR Multiplex Amplification Kit 2400, PN 500022. Included with the complete kit.
Compatible instrumentsInstruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including:
Bio-Rad CFX96 / CFX384 and CFX Opus
Roche LightCycler 480
Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage−20 °C
Shelf lifeUp to 24 months from the date of manufacture, depending on lot and components

Quality

  • Lot tested. Every lot is tested before release.
  • Safety Data Sheet. Available on request from info@bandit.bio.
  • Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
  • Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.

Ordering and pricing

OptionSKUReactionsPriceOrder
Complete kit
Both panels and both master mixes500061 + 500024 + 50002248$2,497
Save $278 vs. buying both kits
À la carte
Panels only: Glyphis Wound Panel A and Pongo Antibiotic Resistance Panel A50006148$1,230
Master mixes only: Kit 3600 and Kit 2400500024 + 50002248$1,517

The complete kit is 10% less than buying the two complete kits separately. Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.