Overview
Xen Cannabis Kit B is a multiplex qPCR kit for microbial detection in cannabis research. It targets the Shiga toxin genes stx1 and stx2 associated with Shiga toxin-producing E. coli (STEC), together with Salmonella and a cannabis internal control.
Targets and dye channels
| Target | Dye |
|---|---|
| STEC 1 (stx1) | ROX |
| STEC 2 (stx2) | ROX |
| Salmonella | FAM |
| Cannabis internal control | HEX |
stx1 and stx2 share the ROX channel. This channel does not distinguish the two targets individually.
Controls included
Pathogen controls for Salmonella, stx1 and stx2, plus a cannabis internal control.
Specifications
| Application | Cannabis microbial research |
|---|---|
| Format | Multiplex qPCR |
| Sample input | Extracted DNA from prepared cannabis samples. Contact us for sample preparation and workflow guidance. |
| Fluorophores | FAM, HEX, ROX |
| qPCR run time | Approximately 60 minutes, excluding sample preparation. |
| Kit size | Contact us for available reaction counts and kit configuration. |
| Larger kits | 500 to 1,000 reaction kits are available as a custom order. Request a quote. |
| Instrument requirements | A qPCR instrument with FAM, HEX, ROX detection channels (or equivalent wavelengths). Contact us to confirm compatibility with your instrument. |
| Master mix and format | Contact us to confirm the master mix and kit components for your workflow. |
Workflow support
The qPCR run follows sample preparation and DNA extraction. Sample preparation is additional to the stated qPCR run time. Contact our Field Applications Specialists for workflow guidance and current product documentation.
Each laboratory should verify performance with its own samples, extraction method and instrument.
Ordering
Pricing, reaction counts, catalog number, current availability and lead time are confirmed in your quote. Please include your sample type, instrument and expected testing volume.