Emerging pathogens
Measles Panel A
Measles virus, including the D8 genotype, in a single tube.
For Research Use Only. Not for use in diagnostic procedures.
Overview
Single-tube RT-qPCR detection of measles virus (Morbillivirus hominis). The assay is formulated to detect the D8 genotype seen in recent U.S. outbreaks and all known circulating strains. Positive controls are included at approximately 10,000 copies/µL.
Targets
Targets
Internal control
Specifications
| Format | RT-qPCR, qualitative multiplex |
|---|---|
| Primer and probe mix | 1 tube |
| Positive controls | 1 tube, included |
| Fluorophores | FAM, ROX |
| Sample input | RNA extracted from nasopharyngeal swab or sputum samples. Performance to be verified by the user. |
| Recommended master mix | SeqOnce™ OneStep RT-qPCR Multiplex Amplification Kit 1800, PN 500030. Sold separately. |
| Compatible instruments | Instruments that detect FAM, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series |
| Storage | −20 °C |
Ordering
| Product | SKU | Reactions |
|---|---|---|
| Measles Panel A | 500039 | 200 |
| SeqOnce™ OneStep RT-qPCR Multiplex Amplification Kit 1800 | 500030 | 200 |
| Panel and master mix bundle | 500039 + 500030 | 200 |
Product information sheets are available on request.