Veterinary

Feline Enteric Pathogen qPCR Panel

11 bacterial, parasitic and viral targets associated with feline enteric infection and diarrhea.

For Research Use Only. Not for use in diagnostic procedures.

Overview

Qualitative multiplex RT-qPCR detection of 11 feline enteric targets across bacteria, parasites and viruses in three tubes. When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.

Targets

Bacteria

Clostridium difficile toxin A/B
Clostridium perfringens enterotoxin (CPE)
Clostridium perfringens alpha toxin (CPA)
Salmonella spp.
Campylobacter (jejuni and coli)

Parasites

Cryptosporidium spp.
Giardia spp.
Tritrichomonas foetus / T. blagburni
Toxoplasma gondii

Viruses

Feline enteric coronavirus
Feline parvovirus 2 (FPV)

Internal control

SPRY1 multi-species control

Specifications

Format	RT-qPCR, qualitative multiplex
Primer and probe mix	3 tubes
Positive controls	Veterinary Enteric Pathogen Positive Control, mixed (PN 450051) or individual tubes (PN 450055, 450056, 450057), included
Fluorophores	FAM, HEX, Cy5, ROX
Sample input	Extracted DNA and RNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user.
Recommended master mix	Bandit Biosystems RT-qPCR Multiplex Amplification Kit 2250, PN 500044. Sold separately.
Compatible instruments	Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage	−20 °C

Tube and dye layout

Tube	FAM	HEX	Cy5	ROX
Tube 1	C. difficile toxin A/B	C. perfringens CPE	C. perfringens CPA	Feline parvovirus 2
Tube 2	SPRY1 control	Salmonella spp.	Cryptosporidium spp.	Campylobacter (jejuni and coli)
Tube 3	Feline enteric coronavirus	Giardia spp.	Tritrichomonas foetus / T. blagburni	Toxoplasma gondii

HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.

Recommended cycling

Reverse transcription: 50 °C for 15 minutes
Enzyme activation: 95 °C for 2 minutes
40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
Collect fluorescence during the 59 °C step

Ordering

Product	SKU	Reactions
Feline Enteric Pathogen qPCR Panel	500054	100
Bandit Biosystems RT-qPCR Multiplex Amplification Kit 2250	500044	100
Panel and master mix bundle	500054 + 500044	100

Product information sheets are available on request.