Veterinary

Canine Enteric Pathogen qPCR Panel

13 bacterial, parasitic and viral targets associated with canine enteric infection and diarrhea.

For Research Use Only. Not for use in diagnostic procedures.

Overview

Qualitative multiplex RT-qPCR detection of 13 canine enteric targets across bacteria, parasites and viruses in four tubes. When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.

Targets

Bacteria

Clostridium difficile toxin A/B
Clostridium perfringens enterotoxin (CPE)
Clostridium perfringens alpha toxin (CPA)
Clostridium perfringens NetE/NetF
Salmonella spp.
Campylobacter (jejuni and coli)
Lawsonia intracellularis

Parasites

Cryptosporidium spp.
Giardia spp.

Viruses

Canine circovirus
Canine distemper virus
Canine enteric coronavirus
Canine parvovirus

Internal control

SPRY1 multi-species control

Specifications

Format	RT-qPCR, qualitative multiplex
Primer and probe mix	4 tubes
Positive controls	Veterinary Enteric Pathogen Positive Control, mixed (PN 450051) or three individual tubes (PN 450055, 450056, 450057), included
Fluorophores	FAM, HEX, Cy5, ROX
Sample input	Extracted DNA and RNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user.
Recommended master mix	Bandit Biosystems RT-qPCR Multiplex Amplification Kit 3000, PN 500055. Sold separately.
Compatible instruments	Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage	−20 °C

Tube and dye layout

Tube	FAM	HEX	Cy5	ROX
Tube 1	C. difficile toxin A/B	C. perfringens CPE	C. perfringens CPA	C. perfringens NetE/NetF
Tube 2	SPRY1 control	Canine circovirus	Canine distemper virus	Canine enteric coronavirus
Tube 3	SPRY1 control	Salmonella spp.	Cryptosporidium spp.	Campylobacter (jejuni and coli)
Tube 4	SPRY1 control	Giardia spp.	Canine parvovirus	Lawsonia intracellularis

HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.

Recommended cycling

Reverse transcription: 50 °C for 15 minutes
Enzyme activation: 95 °C for 2 minutes
40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
Collect fluorescence during the 59 °C step

Ordering

Product	SKU	Reactions
Canine Enteric Pathogen qPCR Panel	500053	100
Bandit Biosystems RT-qPCR Multiplex Amplification Kit 3000	500055	100
Panel and master mix bundle	500053 + 500055	100

Product information sheets are available on request.