Respiratory

AmphiSense™ Respiratory Panel XL

25 respiratory viruses and bacteria, plus mecA.

For Research Use Only. Not for use in diagnostic procedures.

Overview

Expanded RT-qPCR multiplex profiling of respiratory viruses and bacteria. RNA and DNA targets are detected from nasopharyngeal swab and sputum samples in seven tubes.

Targets

Viruses

SARS-CoV-2
Influenza A (pan)
Influenza B
RSV A and B
Human metapneumovirus (HMPV)
Parainfluenza 1–3
Adenovirus
Rhinovirus
Enterovirus
Enterovirus D68
Coronavirus 229E
Coronavirus HKU1
Coronavirus NL63
Coronavirus OC43

Bacteria

Bordetella pertussis
Bordetella parapertussis
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Haemophilus influenzae
Klebsiella pneumoniae
Moraxella catarrhalis
Staphylococcus aureus
Streptococcus pneumoniae
Streptococcus pyogenes

Resistance gene

mecA

Internal control

Human RNase P (hRPP)

Specifications

Format	RT-qPCR, qualitative multiplex
Primer and probe mix	7 tubes
Positive controls	5 tubes, included
Fluorophores	FAM, HEX, Cy5, ROX
Sample input	DNA and RNA extracted from nasopharyngeal swab or sputum samples. Performance to be verified by the user.
Recommended master mix	SeqOnce™ OneStep RT-qPCR Multiplex Amplification Kit 3000, PN 500027. Sold separately.
Compatible instruments	Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage	−20 °C

Ordering

Product	SKU	Reactions
AmphiSense™ Respiratory Panel XL	500004	200
SeqOnce™ OneStep RT-qPCR Multiplex Amplification Kit 3000	500027	200
Panel and master mix bundle	500004 + 500027	200

Product information sheets are available on request.