NGS library prep
NGS library preparation
RhinoSeq™ builds sequencing-ready Illumina DNA libraries in 20 to 40 minutes from as little as 10 ng of DNA, in a single tube. Pair it with D5/D7 dual indexes to multiplex samples on one run.
For Research Use Only. Not for use in diagnostic procedures.
- 20–40 minfrom DNA to library
- 10 ngminimum DNA input
- 1 tubethree steps, one cleanup
- 8 · 24 · 96reaction formats
Products
The NGS line
How RhinoSeq works
Three steps in one tube
- FragmentDNA is fragmented enzymatically, without GC bias.
- LigateA single master mix forms a temporary structure with sequence-specific single-stranded overhangs, so adapters ligate quickly and efficiently. The structure is degraded during the protocol and is not sequenced.
- Index and size-selectLibraries are ready to sequence or to amplify with D5/D7 dual indexes, followed by a single size selection at >180 bp, >300 bp or dual-size.
Why labs use it
Less time at the bench
- Fast. Libraries in 20 to 40 minutes, a fraction of the time of conventional multi-step protocols.
- Fewer transfers. One transfer and cleanup step, compared with three or more in traditional workflows.
- Low input. Start from as little as 10 ng of DNA from any sample source.
- Automatable. A single-tube workflow with less hands-on time, in 8, 24 and 96 reaction formats.
- Made in the USA. Manufactured and supported by Bandit Biosystems.
Specifications
At a glance
| Applications | Whole genome sequencing (WGS). Other applications in development. |
|---|---|
| Platform | Illumina sequencers |
| Sample input | From 10 ng of DNA |
| Time to library | 20 to 40 minutes |
| Size selection | >180 bp, >300 bp and dual-size |
| Indexes | D5/D7 dual indexes, 8, 24 and 96 well, sold separately |
Data and background
Posters and technical notes
- PosterRead
ASHG 2019: a 12-minute, single-tube, nanogram-input library prep for multiple NGS applications
Performance data for RhinoSeq™ library construction across applications.
- PosterRead
ABRF 2019: a 12-minute, single-tube, nanogram-input library prep
Workflow and library quality results for RhinoSeq™.
- Technical noteRead
Understanding NGS library complexity: sources of sequencing errors and limits of detection
How library complexity affects error rates and detection limits.
- Press releaseRead
RhinoSeq™ rapid DNA library preparation kit launch, March 2019
The original SeqOnce Biosciences launch announcement, kept in our archive.
Planning a sequencing project?
Ask about kit sizes, index sets, bulk or OEM supply, or how RhinoSeq™ fits your workflow.